Sequence Submission Portal

An overall goal of this portal is to establish a large agricultural genomic sequence database at national level. This system will provide an extensive vocabulary describing features of the genomic data in the field of bioinformatics and a useful platform for extracting useful information.

Features of the portal:

  • Submitter is required to register on the portal. The submitter's profile information is saved on our database. Each subsequent time the submitter can login to the system by entering login id and password.
  • It allows the submitter to submit a single sequence at a time
  • For submitting multiple sequence use our "Bulk Submission" link on the home page.
  • This portal accepts sequence data both as text input and as uploaded files.
  • It also saves the history of submissions made by the user marked as "Completed Submission".

Options for Submission

Go for using this portal in the following situations:
  • If you have a single sequence and you prefer to use a web-based submission tool..
  • Use our "Bulk Submission" link, if you prefer to work on your submission off-line you have a sequence or sequences that are complex.

How to use this portal:

  • This portal is mainly aimed for the researchers who are working in agricultural bioinformatics.
  • Paste the sequence in the query box with single letter IUPAC (International Union of pure and applied Chemistry) code.
  • Specify the organelle location, source modifiers, and submitter­s name with various features and options of functional elements of a species.

Requirements for Sequence Submissions through dnadb

  • Contact Information
    •
    • Name, address, phone number, fax number and email address of the submitter must be entered when registering and submitting for the first time
    • Subsequent dnadb submissions will retain this information and display it once the submitter logs in
  • Release date information
    •
    • Immediately after it is processed at NCBI OR
    • On a date the submitter specifies
  • Reference information
    •
    • Sequence authors: names of the researchers who are credited with the sequence
    • Publication information: Unpublished, In-Press, or Published; and applicable citation information (paper's title, authors, journal title, volume, issue, year, pages)
  • Sequencing Technology
    •
    • This information is required if you are submitting over 500 sequences or if your sequences were generated using next-generation sequencing technology.
    • What methods were used to obtain these sequences?
  • Submission Category and Type
    •
    • Original sequencing or Third Party Annotation
  • Nucleotide sequence(s)
    •
    • Sequence data in the FASTA format can be either cut-and-pasted as text or uploaded from the local folder.
    • Sequences must be at least 200 nucleotides long.
    • Molecule type: what was sequenced? (genomic DNA, mRNA, genomic RNA, cRNA, etc)
    • Topology: linear or circular (circular must be complete, such as a complete plasmid)
  • Organism name, applicable source modifiers, location
    •
    • Source organism name (an appropriate scientific name for the source organism is crucial for proper taxonomic assignment and genetic code use for conceptual translation of coding regions).
    • Most complete name for any synthetic vector (for example: Cloning vector pAB234, Transfer vector p789Abc)
    • Source modifiers include: strain, clone, isolate, specimen-voucher, isolation-source, country
    • Location: organelle (mitochondrion, chloroplast, etc); map and/or chromosome
  • Features of the sequence
    •
    • use input forms to add all applicable features (for example: CDS, gene, rRNA, tRNA, microsatellite, exon, intron)