PRIMER_THERMODYNAMIC_ALIGNMENT=1
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=../primer3_config/
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_DIVALENT=1.5
P3_COMMENT=The next line to reproduce behavior with -default_version=1
PRIMER_DNTP_CONC=0.0
PRIMER_FIRST_BASE_INDEX=1
PRIMER_MAX_SELF_ANY=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_PAIR_MAX_COMPL_END=8
P3_FILE_FLAG=1
SEQUENCE_ID=targets_test
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
SEQUENCE_TARGET=22,122
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=SP01-02.txt
SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.564009
PRIMER_LEFT_0_PENALTY=2.796318
PRIMER_RIGHT_0_PENALTY=0.767691
PRIMER_LEFT_0_SEQUENCE=GCTGTTTCACACTTACGGAA
PRIMER_RIGHT_0_SEQUENCE=TGTTGGAGAAAAGCCACTGG
PRIMER_LEFT_0=8,20
PRIMER_RIGHT_0=245,20
PRIMER_LEFT_0_TM=57.204
PRIMER_RIGHT_0_TM=59.232
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=35.22
PRIMER_LEFT_0_END_STABILITY=4.3000
PRIMER_RIGHT_0_END_STABILITY=4.0000
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.32
PRIMER_PAIR_0_PRODUCT_SIZE=238
PRIMER_PAIR_1_PENALTY=3.818870
PRIMER_LEFT_1_PENALTY=3.051179
PRIMER_RIGHT_1_PENALTY=0.767691
PRIMER_LEFT_1_SEQUENCE=GCTGTTTCACACTTACGGAAA
PRIMER_RIGHT_1_SEQUENCE=TGTTGGAGAAAAGCCACTGG
PRIMER_LEFT_1=8,21
PRIMER_RIGHT_1=245,20
PRIMER_LEFT_1_TM=57.949
PRIMER_RIGHT_1_TM=59.232
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=35.22
PRIMER_LEFT_1_END_STABILITY=3.1300
PRIMER_RIGHT_1_END_STABILITY=4.0000
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.32
PRIMER_PAIR_1_PRODUCT_SIZE=238
PRIMER_PAIR_2_PENALTY=3.853878
PRIMER_LEFT_2_PENALTY=2.796318
PRIMER_RIGHT_2_PENALTY=1.057560
PRIMER_LEFT_2_SEQUENCE=GCTGTTTCACACTTACGGAA
PRIMER_RIGHT_2_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_LEFT_2=8,20
PRIMER_RIGHT_2=248,20
PRIMER_LEFT_2_TM=57.204
PRIMER_RIGHT_2_TM=58.942
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=38.11
PRIMER_LEFT_2_END_STABILITY=4.3000
PRIMER_RIGHT_2_END_STABILITY=5.0100
PRIMER_PAIR_2_COMPL_ANY_TH=4.27
PRIMER_PAIR_2_COMPL_END_TH=4.03
PRIMER_PAIR_2_PRODUCT_SIZE=241
PRIMER_PAIR_3_PENALTY=3.857112
PRIMER_LEFT_3_PENALTY=2.796318
PRIMER_RIGHT_3_PENALTY=1.060794
PRIMER_LEFT_3_SEQUENCE=GCTGTTTCACACTTACGGAA
PRIMER_RIGHT_3_SEQUENCE=CCTGTTGGAGAAAAGCCACT
PRIMER_LEFT_3=8,20
PRIMER_RIGHT_3=247,20
PRIMER_LEFT_3_TM=57.204
PRIMER_RIGHT_3_TM=58.939
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=35.22
PRIMER_LEFT_3_END_STABILITY=4.3000
PRIMER_RIGHT_3_END_STABILITY=4.0000
PRIMER_PAIR_3_COMPL_ANY_TH=3.04
PRIMER_PAIR_3_COMPL_END_TH=2.28
PRIMER_PAIR_3_PRODUCT_SIZE=240
PRIMER_PAIR_4_PENALTY=4.080246
PRIMER_LEFT_4_PENALTY=3.312555
PRIMER_RIGHT_4_PENALTY=0.767691
PRIMER_LEFT_4_SEQUENCE=GCTGTTTCACACTTACGGAAATG
PRIMER_RIGHT_4_SEQUENCE=TGTTGGAGAAAAGCCACTGG
PRIMER_LEFT_4=8,23
PRIMER_RIGHT_4=245,20
PRIMER_LEFT_4_TM=59.687
PRIMER_RIGHT_4_TM=59.232
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=35.22
PRIMER_LEFT_4_END_STABILITY=2.3200
PRIMER_RIGHT_4_END_STABILITY=4.0000
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.32
PRIMER_PAIR_4_PRODUCT_SIZE=238
=
SEQUENCE_ID=SP01-03.txt
SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.582080
PRIMER_LEFT_0_PENALTY=0.199248
PRIMER_RIGHT_0_PENALTY=0.382831
PRIMER_LEFT_0_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
PRIMER_RIGHT_0_SEQUENCE=AAGCAAATGGACGTGGCATC
PRIMER_LEFT_0=66,20
PRIMER_RIGHT_0=165,20
PRIMER_LEFT_0_TM=59.801
PRIMER_RIGHT_0_TM=60.383
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=4.1600
PRIMER_RIGHT_0_END_STABILITY=3.9100
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.582080
PRIMER_LEFT_1_PENALTY=0.199248
PRIMER_RIGHT_1_PENALTY=0.382831
PRIMER_LEFT_1_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
PRIMER_RIGHT_1_SEQUENCE=GAAGCAAATGGACGTGGCAT
PRIMER_LEFT_1=66,20
PRIMER_RIGHT_1=166,20
PRIMER_LEFT_1_TM=59.801
PRIMER_RIGHT_1_TM=60.383
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_END_STABILITY=4.1600
PRIMER_RIGHT_1_END_STABILITY=4.4000
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=101
PRIMER_PAIR_2_PENALTY=0.625979
PRIMER_LEFT_2_PENALTY=0.243148
PRIMER_RIGHT_2_PENALTY=0.382831
PRIMER_LEFT_2_SEQUENCE=CCTGCAGGTTTAGAAGGGGT
PRIMER_RIGHT_2_SEQUENCE=AAGCAAATGGACGTGGCATC
PRIMER_LEFT_2=64,20
PRIMER_RIGHT_2=165,20
PRIMER_LEFT_2_TM=60.243
PRIMER_RIGHT_2_TM=60.383
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY_TH=26.16
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_END_STABILITY=4.9500
PRIMER_RIGHT_2_END_STABILITY=3.9100
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=102
PRIMER_PAIR_3_PENALTY=0.625979
PRIMER_LEFT_3_PENALTY=0.243148
PRIMER_RIGHT_3_PENALTY=0.382831
PRIMER_LEFT_3_SEQUENCE=CCTGCAGGTTTAGAAGGGGT
PRIMER_RIGHT_3_SEQUENCE=GAAGCAAATGGACGTGGCAT
PRIMER_LEFT_3=64,20
PRIMER_RIGHT_3=166,20
PRIMER_LEFT_3_TM=60.243
PRIMER_RIGHT_3_TM=60.383
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY_TH=26.16
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_END_STABILITY=4.9500
PRIMER_RIGHT_3_END_STABILITY=4.4000
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=103
PRIMER_PAIR_4_PENALTY=0.734986
PRIMER_LEFT_4_PENALTY=0.423041
PRIMER_RIGHT_4_PENALTY=0.311945
PRIMER_LEFT_4_SEQUENCE=ATCTGCACCCTGTAAACCCT
PRIMER_RIGHT_4_SEQUENCE=GTCACCAAATGCACCCAGAC
PRIMER_LEFT_4=3,20
PRIMER_RIGHT_4=103,20
PRIMER_LEFT_4_TM=59.577
PRIMER_RIGHT_4_TM=60.312
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_END_STABILITY=4.3400
PRIMER_RIGHT_4_END_STABILITY=3.5100
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=101
=
SEQUENCE_ID=SP01-06.txt
SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.875269
PRIMER_LEFT_0_PENALTY=6.659117
PRIMER_RIGHT_0_PENALTY=2.216152
PRIMER_LEFT_0_SEQUENCE=TCGCAATCAAATTTTTTCTATGACCT
PRIMER_RIGHT_0_SEQUENCE=GATCGGGAGCGTGCTTTG
PRIMER_LEFT_0=37,26
PRIMER_RIGHT_0=136,18
PRIMER_LEFT_0_TM=59.341
PRIMER_RIGHT_0_TM=59.784
PRIMER_LEFT_0_GC_PERCENT=30.769
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=3.8500
PRIMER_RIGHT_0_END_STABILITY=2.7700
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=9.102381
PRIMER_LEFT_1_PENALTY=6.886230
PRIMER_RIGHT_1_PENALTY=2.216152
PRIMER_LEFT_1_SEQUENCE=TCGCAATCAAATTTTTTCTATGACC
PRIMER_RIGHT_1_SEQUENCE=GATCGGGAGCGTGCTTTG
PRIMER_LEFT_1=37,25
PRIMER_RIGHT_1=136,18
PRIMER_LEFT_1_TM=58.114
PRIMER_RIGHT_1_TM=59.784
PRIMER_LEFT_1_GC_PERCENT=32.000
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_END_STABILITY=4.0200
PRIMER_RIGHT_1_END_STABILITY=2.7700
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=9.521198
PRIMER_LEFT_2_PENALTY=7.305046
PRIMER_RIGHT_2_PENALTY=2.216152
PRIMER_LEFT_2_SEQUENCE=ATCGCAATCAAATTTTTTCTATGACCT
PRIMER_RIGHT_2_SEQUENCE=GATCGGGAGCGTGCTTTG
PRIMER_LEFT_2=36,27
PRIMER_RIGHT_2=136,18
PRIMER_LEFT_2_TM=59.695
PRIMER_RIGHT_2_TM=59.784
PRIMER_LEFT_2_GC_PERCENT=29.630
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_END_STABILITY=3.8500
PRIMER_RIGHT_2_END_STABILITY=2.7700
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=101
PRIMER_PAIR_3_PENALTY=9.610505
PRIMER_LEFT_3_PENALTY=7.394353
PRIMER_RIGHT_3_PENALTY=2.216152
PRIMER_LEFT_3_SEQUENCE=TCGCAATCAAATTTTTTCTATGACCTG
PRIMER_RIGHT_3_SEQUENCE=GATCGGGAGCGTGCTTTG
PRIMER_LEFT_3=37,27
PRIMER_RIGHT_3=136,18
PRIMER_LEFT_3_TM=60.394
PRIMER_RIGHT_3_TM=59.784
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=61.111
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_END_STABILITY=4.0000
PRIMER_RIGHT_3_END_STABILITY=2.7700
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=9.696873
PRIMER_LEFT_4_PENALTY=7.480721
PRIMER_RIGHT_4_PENALTY=2.216152
PRIMER_LEFT_4_SEQUENCE=ATCGCAATCAAATTTTTTCTATGACC
PRIMER_RIGHT_4_SEQUENCE=GATCGGGAGCGTGCTTTG
PRIMER_LEFT_4=36,26
PRIMER_RIGHT_4=136,18
PRIMER_LEFT_4_TM=58.519
PRIMER_RIGHT_4_TM=59.784
PRIMER_LEFT_4_GC_PERCENT=30.769
PRIMER_RIGHT_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_END_STABILITY=4.0200
PRIMER_RIGHT_4_END_STABILITY=2.7700
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=101
=
SEQUENCE_ID=SP01-15.txt
SEQUENCE_TEMPLATE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=SP01-19.txt
SEQUENCE_TEMPLATE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.078669
PRIMER_LEFT_0_PENALTY=0.048989
PRIMER_RIGHT_0_PENALTY=0.029680
PRIMER_LEFT_0_SEQUENCE=AACCTGGCTACAGTTCCCTC
PRIMER_RIGHT_0_SEQUENCE=CAGTGCTCTTTCTGCCAGTG
PRIMER_LEFT_0=72,20
PRIMER_RIGHT_0=176,20
PRIMER_LEFT_0_TM=59.951
PRIMER_RIGHT_0_TM=60.030
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=43.93
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=4.3000
PRIMER_RIGHT_0_END_STABILITY=3.6600
PRIMER_PAIR_0_COMPL_ANY_TH=2.45
PRIMER_PAIR_0_COMPL_END_TH=2.46
PRIMER_PAIR_0_PRODUCT_SIZE=105
PRIMER_PAIR_1_PENALTY=0.078961
PRIMER_LEFT_1_PENALTY=0.048989
PRIMER_RIGHT_1_PENALTY=0.029971
PRIMER_LEFT_1_SEQUENCE=AACCTGGCTACAGTTCCCTC
PRIMER_RIGHT_1_SEQUENCE=GCTCTTTCTGCCAGTGTCAC
PRIMER_LEFT_1=72,20
PRIMER_RIGHT_1=172,20
PRIMER_LEFT_1_TM=59.951
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=43.93
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_END_STABILITY=4.3000
PRIMER_RIGHT_1_END_STABILITY=3.6700
PRIMER_PAIR_1_COMPL_ANY_TH=5.96
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=101
PRIMER_PAIR_2_PENALTY=0.078961
PRIMER_LEFT_2_PENALTY=0.048989
PRIMER_RIGHT_2_PENALTY=0.029971
PRIMER_LEFT_2_SEQUENCE=AACCTGGCTACAGTTCCCTC
PRIMER_RIGHT_2_SEQUENCE=GTGCTCTTTCTGCCAGTGTC
PRIMER_LEFT_2=72,20
PRIMER_RIGHT_2=174,20
PRIMER_LEFT_2_TM=59.951
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=43.93
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_END_STABILITY=4.3000
PRIMER_RIGHT_2_END_STABILITY=3.6700
PRIMER_PAIR_2_COMPL_ANY_TH=5.96
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=103
PRIMER_PAIR_3_PENALTY=0.233107
PRIMER_LEFT_3_PENALTY=0.048989
PRIMER_RIGHT_3_PENALTY=0.184118
PRIMER_LEFT_3_SEQUENCE=AACCTGGCTACAGTTCCCTC
PRIMER_RIGHT_3_SEQUENCE=CATCCAGTGCTCTTTCTGCC
PRIMER_LEFT_3=72,20
PRIMER_RIGHT_3=180,20
PRIMER_LEFT_3_TM=59.951
PRIMER_RIGHT_3_TM=59.816
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=43.93
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_END_STABILITY=4.3000
PRIMER_RIGHT_3_END_STABILITY=4.8500
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=109
PRIMER_PAIR_4_PENALTY=0.291862
PRIMER_LEFT_4_PENALTY=0.048989
PRIMER_RIGHT_4_PENALTY=0.242873
PRIMER_LEFT_4_SEQUENCE=AACCTGGCTACAGTTCCCTC
PRIMER_RIGHT_4_SEQUENCE=ATCCAGTGCTCTTTCTGCCA
PRIMER_LEFT_4=72,20
PRIMER_RIGHT_4=179,20
PRIMER_LEFT_4_TM=59.951
PRIMER_RIGHT_4_TM=60.243
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=43.93
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_END_STABILITY=4.3000
PRIMER_RIGHT_4_END_STABILITY=4.9200
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=108
=
SEQUENCE_ID=SP01-20.txt
SEQUENCE_TEMPLATE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=SP01-21.txt
SEQUENCE_TEMPLATE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.710044
PRIMER_LEFT_0_PENALTY=0.024450
PRIMER_RIGHT_0_PENALTY=0.685594
PRIMER_LEFT_0_SEQUENCE=TGTGTCTTGTGGGTGTGTCT
PRIMER_RIGHT_0_SEQUENCE=ACACACACACACAACACACA
PRIMER_LEFT_0=59,20
PRIMER_RIGHT_0=159,20
PRIMER_LEFT_0_TM=60.024
PRIMER_RIGHT_0_TM=59.314
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=3.4100
PRIMER_RIGHT_0_END_STABILITY=3.7200
PRIMER_PAIR_0_COMPL_ANY_TH=40.01
PRIMER_PAIR_0_COMPL_END_TH=38.93
PRIMER_PAIR_0_PRODUCT_SIZE=101
PRIMER_PAIR_1_PENALTY=1.080122
PRIMER_LEFT_1_PENALTY=0.394528
PRIMER_RIGHT_1_PENALTY=0.685594
PRIMER_LEFT_1_SEQUENCE=GTGTCTTGTGGGTGTGTCTG
PRIMER_RIGHT_1_SEQUENCE=ACACACACACACAACACACA
PRIMER_LEFT_1=60,20
PRIMER_RIGHT_1=159,20
PRIMER_LEFT_1_TM=59.605
PRIMER_RIGHT_1_TM=59.314
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_END_STABILITY=3.5100
PRIMER_RIGHT_1_END_STABILITY=3.7200
PRIMER_PAIR_1_COMPL_ANY_TH=38.19
PRIMER_PAIR_1_COMPL_END_TH=35.05
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=1.129499
PRIMER_LEFT_2_PENALTY=0.024450
PRIMER_RIGHT_2_PENALTY=1.105049
PRIMER_LEFT_2_SEQUENCE=TGTCTTGTGGGTGTGTCTGT
PRIMER_RIGHT_2_SEQUENCE=AGACACACACACACAACACAC
PRIMER_LEFT_2=61,20
PRIMER_RIGHT_2=161,21
PRIMER_LEFT_2_TM=60.024
PRIMER_RIGHT_2_TM=60.105
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_END_STABILITY=3.4100
PRIMER_RIGHT_2_END_STABILITY=3.8200
PRIMER_PAIR_2_COMPL_ANY_TH=31.60
PRIMER_PAIR_2_COMPL_END_TH=34.49
PRIMER_PAIR_2_PRODUCT_SIZE=101
PRIMER_PAIR_3_PENALTY=1.129499
PRIMER_LEFT_3_PENALTY=0.024450
PRIMER_RIGHT_3_PENALTY=1.105049
PRIMER_LEFT_3_SEQUENCE=TGTCTTGTGGGTGTGTCTGT
PRIMER_RIGHT_3_SEQUENCE=ACAGACACACACACACAACAC
PRIMER_LEFT_3=61,20
PRIMER_RIGHT_3=163,21
PRIMER_LEFT_3_TM=60.024
PRIMER_RIGHT_3_TM=60.105
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_END_STABILITY=3.4100
PRIMER_RIGHT_3_END_STABILITY=3.3200
PRIMER_PAIR_3_COMPL_ANY_TH=33.14
PRIMER_PAIR_3_COMPL_END_TH=35.99
PRIMER_PAIR_3_PRODUCT_SIZE=103
PRIMER_PAIR_4_PENALTY=1.129499
PRIMER_LEFT_4_PENALTY=0.024450
PRIMER_RIGHT_4_PENALTY=1.105049
PRIMER_LEFT_4_SEQUENCE=TGTCTTGTGGGTGTGTCTGT
PRIMER_RIGHT_4_SEQUENCE=ACACAACAGACACACACACAC
PRIMER_LEFT_4=61,20
PRIMER_RIGHT_4=168,21
PRIMER_LEFT_4_TM=60.024
PRIMER_RIGHT_4_TM=60.105
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_END_STABILITY=3.4100
PRIMER_RIGHT_4_END_STABILITY=3.8200
PRIMER_PAIR_4_COMPL_ANY_TH=43.66
PRIMER_PAIR_4_COMPL_END_TH=43.66
PRIMER_PAIR_4_PRODUCT_SIZE=108
=
SEQUENCE_ID=SP03-40.txt
SEQUENCE_TEMPLATE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=SP04-07.txt
SEQUENCE_TEMPLATE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.058082
PRIMER_LEFT_0_PENALTY=0.033010
PRIMER_RIGHT_0_PENALTY=0.025072
PRIMER_LEFT_0_SEQUENCE=CCGTGTACACACTGAACACG
PRIMER_RIGHT_0_SEQUENCE=TGTGGCTAGTCATGGAGGTG
PRIMER_LEFT_0=48,20
PRIMER_RIGHT_0=254,20
PRIMER_LEFT_0_TM=60.033
PRIMER_RIGHT_0_TM=60.025
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=25.52
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=20.62
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=44.85
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=4.4900
PRIMER_RIGHT_0_END_STABILITY=4.0000
PRIMER_PAIR_0_COMPL_ANY_TH=1.26
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=207
PRIMER_PAIR_1_PENALTY=0.080334
PRIMER_LEFT_1_PENALTY=0.033010
PRIMER_RIGHT_1_PENALTY=0.047323
PRIMER_LEFT_1_SEQUENCE=CCGTGTACACACTGAACACG
PRIMER_RIGHT_1_SEQUENCE=TCCCACAGCTGTAGGAAGTG
PRIMER_LEFT_1=48,20
PRIMER_RIGHT_1=216,20
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=59.953
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY_TH=25.52
PRIMER_RIGHT_1_SELF_ANY_TH=18.98
PRIMER_LEFT_1_SELF_END_TH=20.62
PRIMER_RIGHT_1_SELF_END_TH=7.67
PRIMER_LEFT_1_HAIRPIN_TH=44.85
PRIMER_RIGHT_1_HAIRPIN_TH=33.99
PRIMER_LEFT_1_END_STABILITY=4.4900
PRIMER_RIGHT_1_END_STABILITY=3.1600
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=169
PRIMER_PAIR_2_PENALTY=0.081847
PRIMER_LEFT_2_PENALTY=0.033010
PRIMER_RIGHT_2_PENALTY=0.048837
PRIMER_LEFT_2_SEQUENCE=CCGTGTACACACTGAACACG
PRIMER_RIGHT_2_SEQUENCE=TCTCCTAGCTGGGTTTGTGG
PRIMER_LEFT_2=48,20
PRIMER_RIGHT_2=269,20
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.951
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY_TH=25.52
PRIMER_RIGHT_2_SELF_ANY_TH=11.17
PRIMER_LEFT_2_SELF_END_TH=20.62
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=44.85
PRIMER_RIGHT_2_HAIRPIN_TH=36.53
PRIMER_LEFT_2_END_STABILITY=4.4900
PRIMER_RIGHT_2_END_STABILITY=4.1700
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=222
PRIMER_PAIR_3_PENALTY=0.132102
PRIMER_LEFT_3_PENALTY=0.033010
PRIMER_RIGHT_3_PENALTY=0.099091
PRIMER_LEFT_3_SEQUENCE=CCGTGTACACACTGAACACG
PRIMER_RIGHT_3_SEQUENCE=GAGCCTCATTTTCCCACAGC
PRIMER_LEFT_3=48,20
PRIMER_RIGHT_3=227,20
PRIMER_LEFT_3_TM=60.033
PRIMER_RIGHT_3_TM=60.099
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY_TH=25.52
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=20.62
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=44.85
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_END_STABILITY=4.4900
PRIMER_RIGHT_3_END_STABILITY=4.4000
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=180
PRIMER_PAIR_4_PENALTY=0.205019
PRIMER_LEFT_4_PENALTY=0.179947
PRIMER_RIGHT_4_PENALTY=0.025072
PRIMER_LEFT_4_SEQUENCE=CCGCCATGTACACACTGAAG
PRIMER_RIGHT_4_SEQUENCE=TGTGGCTAGTCATGGAGGTG
PRIMER_LEFT_4=96,20
PRIMER_RIGHT_4=254,20
PRIMER_LEFT_4_TM=59.820
PRIMER_RIGHT_4_TM=60.025
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_END_STABILITY=3.0200
PRIMER_RIGHT_4_END_STABILITY=4.0000
PRIMER_PAIR_4_COMPL_ANY_TH=16.34
PRIMER_PAIR_4_COMPL_END_TH=3.49
PRIMER_PAIR_4_PRODUCT_SIZE=159
=
SEQUENCE_ID=AGAG
SEQUENCE_TEMPLATE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=long_seq
SEQUENCE_TEMPLATE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.128531
PRIMER_LEFT_0_PENALTY=0.028008
PRIMER_RIGHT_0_PENALTY=0.100522
PRIMER_LEFT_0_SEQUENCE=tgctagctaggcgatgctag
PRIMER_RIGHT_0_SEQUENCE=ccgctacgatcgatcagcta
PRIMER_LEFT_0=411,20
PRIMER_RIGHT_0=584,20
PRIMER_LEFT_0_TM=60.028
PRIMER_RIGHT_0_TM=60.101
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=23.16
PRIMER_RIGHT_0_SELF_ANY_TH=26.08
PRIMER_LEFT_0_SELF_END_TH=23.16
PRIMER_RIGHT_0_SELF_END_TH=14.12
PRIMER_LEFT_0_HAIRPIN_TH=38.59
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=3.4200
PRIMER_RIGHT_0_END_STABILITY=3.3200
PRIMER_PAIR_0_COMPL_ANY_TH=7.52
PRIMER_PAIR_0_COMPL_END_TH=6.37
PRIMER_PAIR_0_PRODUCT_SIZE=174
PRIMER_PAIR_1_PENALTY=0.143566
PRIMER_LEFT_1_PENALTY=0.043044
PRIMER_RIGHT_1_PENALTY=0.100522
PRIMER_LEFT_1_SEQUENCE=actgatacgcgatgctagct
PRIMER_RIGHT_1_SEQUENCE=ccgctacgatcgatcagcta
PRIMER_LEFT_1=476,20
PRIMER_RIGHT_1=584,20
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=60.101
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY_TH=17.69
PRIMER_RIGHT_1_SELF_ANY_TH=26.08
PRIMER_LEFT_1_SELF_END_TH=1.35
PRIMER_RIGHT_1_SELF_END_TH=14.12
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_END_STABILITY=3.3200
PRIMER_RIGHT_1_END_STABILITY=3.3200
PRIMER_PAIR_1_COMPL_ANY_TH=15.45
PRIMER_PAIR_1_COMPL_END_TH=2.99
PRIMER_PAIR_1_PRODUCT_SIZE=109
PRIMER_PAIR_2_PENALTY=0.200309
PRIMER_LEFT_2_PENALTY=0.099786
PRIMER_RIGHT_2_PENALTY=0.100522
PRIMER_LEFT_2_SEQUENCE=gatcgatgctagctaggcga
PRIMER_RIGHT_2_SEQUENCE=ccgctacgatcgatcagcta
PRIMER_LEFT_2=405,20
PRIMER_RIGHT_2=584,20
PRIMER_LEFT_2_TM=60.100
PRIMER_RIGHT_2_TM=60.101
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY_TH=16.30
PRIMER_RIGHT_2_SELF_ANY_TH=26.08
PRIMER_LEFT_2_SELF_END_TH=16.30
PRIMER_RIGHT_2_SELF_END_TH=14.12
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_END_STABILITY=5.5400
PRIMER_RIGHT_2_END_STABILITY=3.3200
PRIMER_PAIR_2_COMPL_ANY_TH=18.59
PRIMER_PAIR_2_COMPL_END_TH=18.43
PRIMER_PAIR_2_PRODUCT_SIZE=180
PRIMER_PAIR_3_PENALTY=0.200309
PRIMER_LEFT_3_PENALTY=0.099786
PRIMER_RIGHT_3_PENALTY=0.100522
PRIMER_LEFT_3_SEQUENCE=tcgatcgatgctagctaggc
PRIMER_RIGHT_3_SEQUENCE=ccgctacgatcgatcagcta
PRIMER_LEFT_3=403,20
PRIMER_RIGHT_3=584,20
PRIMER_LEFT_3_TM=60.100
PRIMER_RIGHT_3_TM=60.101
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY_TH=18.63
PRIMER_RIGHT_3_SELF_ANY_TH=26.08
PRIMER_LEFT_3_SELF_END_TH=8.45
PRIMER_RIGHT_3_SELF_END_TH=14.12
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_END_STABILITY=3.9300
PRIMER_RIGHT_3_END_STABILITY=3.3200
PRIMER_PAIR_3_COMPL_ANY_TH=22.41
PRIMER_PAIR_3_COMPL_END_TH=19.54
PRIMER_PAIR_3_PRODUCT_SIZE=182
PRIMER_PAIR_4_PENALTY=0.295718
PRIMER_LEFT_4_PENALTY=0.252674
PRIMER_RIGHT_4_PENALTY=0.043044
PRIMER_LEFT_4_SEQUENCE=tatcatctctgcgcgatcga
PRIMER_RIGHT_4_SEQUENCE=agctagcatcgcgtatcagt
PRIMER_LEFT_4=361,20
PRIMER_RIGHT_4=495,20
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=59.957
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY_TH=22.07
PRIMER_RIGHT_4_SELF_ANY_TH=19.30
PRIMER_LEFT_4_SELF_END_TH=1.72
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=38.48
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_END_STABILITY=3.5900
PRIMER_RIGHT_4_END_STABILITY=3.4100
PRIMER_PAIR_4_COMPL_ANY_TH=15.85
PRIMER_PAIR_4_COMPL_END_TH=7.27
PRIMER_PAIR_4_PRODUCT_SIZE=135
=
SEQUENCE_ID=bad_letter
SEQUENCE_TEMPLATE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC
PRIMER_ERROR=Unrecognized base in input sequence
=
SEQUENCE_ID=bad_target
SEQUENCE_TEMPLATE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC
SEQUENCE_TARGET=51,10
SEQUENCE_TARGET=501,50
PRIMER_ERROR=TARGET beyond end of sequence
=
SEQUENCE_ID=other_bad_target
SEQUENCE_TEMPLATE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC
SEQUENCE_TARGET=101,1000
PRIMER_ERROR=TARGET beyond end of sequence
=
SEQUENCE_ID=included_test
SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
SEQUENCE_INCLUDED_REGION=21,228
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.710044
PRIMER_LEFT_0_PENALTY=0.024450
PRIMER_RIGHT_0_PENALTY=0.685594
PRIMER_LEFT_0_SEQUENCE=TGTGTCTTGTGGGTGTGTCT
PRIMER_RIGHT_0_SEQUENCE=ACACACACACACAACACACA
PRIMER_LEFT_0=79,20
PRIMER_RIGHT_0=179,20
PRIMER_LEFT_0_TM=60.024
PRIMER_RIGHT_0_TM=59.314
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=3.4100
PRIMER_RIGHT_0_END_STABILITY=3.7200
PRIMER_PAIR_0_COMPL_ANY_TH=40.01
PRIMER_PAIR_0_COMPL_END_TH=38.93
PRIMER_PAIR_0_PRODUCT_SIZE=101
PRIMER_PAIR_1_PENALTY=1.080122
PRIMER_LEFT_1_PENALTY=0.394528
PRIMER_RIGHT_1_PENALTY=0.685594
PRIMER_LEFT_1_SEQUENCE=GTGTCTTGTGGGTGTGTCTG
PRIMER_RIGHT_1_SEQUENCE=ACACACACACACAACACACA
PRIMER_LEFT_1=80,20
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=59.605
PRIMER_RIGHT_1_TM=59.314
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_END_STABILITY=3.5100
PRIMER_RIGHT_1_END_STABILITY=3.7200
PRIMER_PAIR_1_COMPL_ANY_TH=38.19
PRIMER_PAIR_1_COMPL_END_TH=35.05
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=1.129499
PRIMER_LEFT_2_PENALTY=0.024450
PRIMER_RIGHT_2_PENALTY=1.105049
PRIMER_LEFT_2_SEQUENCE=TGTCTTGTGGGTGTGTCTGT
PRIMER_RIGHT_2_SEQUENCE=AGACACACACACACAACACAC
PRIMER_LEFT_2=81,20
PRIMER_RIGHT_2=181,21
PRIMER_LEFT_2_TM=60.024
PRIMER_RIGHT_2_TM=60.105
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_END_STABILITY=3.4100
PRIMER_RIGHT_2_END_STABILITY=3.8200
PRIMER_PAIR_2_COMPL_ANY_TH=31.60
PRIMER_PAIR_2_COMPL_END_TH=34.49
PRIMER_PAIR_2_PRODUCT_SIZE=101
PRIMER_PAIR_3_PENALTY=1.129499
PRIMER_LEFT_3_PENALTY=0.024450
PRIMER_RIGHT_3_PENALTY=1.105049
PRIMER_LEFT_3_SEQUENCE=TGTCTTGTGGGTGTGTCTGT
PRIMER_RIGHT_3_SEQUENCE=ACAGACACACACACACAACAC
PRIMER_LEFT_3=81,20
PRIMER_RIGHT_3=183,21
PRIMER_LEFT_3_TM=60.024
PRIMER_RIGHT_3_TM=60.105
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_END_STABILITY=3.4100
PRIMER_RIGHT_3_END_STABILITY=3.3200
PRIMER_PAIR_3_COMPL_ANY_TH=33.14
PRIMER_PAIR_3_COMPL_END_TH=35.99
PRIMER_PAIR_3_PRODUCT_SIZE=103
PRIMER_PAIR_4_PENALTY=1.129499
PRIMER_LEFT_4_PENALTY=0.024450
PRIMER_RIGHT_4_PENALTY=1.105049
PRIMER_LEFT_4_SEQUENCE=TGTCTTGTGGGTGTGTCTGT
PRIMER_RIGHT_4_SEQUENCE=ACACAACAGACACACACACAC
PRIMER_LEFT_4=81,20
PRIMER_RIGHT_4=188,21
PRIMER_LEFT_4_TM=60.024
PRIMER_RIGHT_4_TM=60.105
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_END_STABILITY=3.4100
PRIMER_RIGHT_4_END_STABILITY=3.8200
PRIMER_PAIR_4_COMPL_ANY_TH=43.66
PRIMER_PAIR_4_COMPL_END_TH=43.66
PRIMER_PAIR_4_PRODUCT_SIZE=108
=
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_MAX_SELF_END=8
SEQUENCE_ID=included_test_w_middle
SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
SEQUENCE_INCLUDED_REGION=21,228
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.286428
PRIMER_LEFT_0_PENALTY=0.024450
PRIMER_RIGHT_0_PENALTY=1.261978
PRIMER_INTERNAL_0_PENALTY=9.546432
PRIMER_LEFT_0_SEQUENCE=TGTCTTGTGGGTGTGTCTGT
PRIMER_RIGHT_0_SEQUENCE=ACACAACAGACACACACACA
PRIMER_INTERNAL_0_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG
PRIMER_LEFT_0=81,20
PRIMER_RIGHT_0=188,20
PRIMER_INTERNAL_0=142,27
PRIMER_LEFT_0_TM=60.024
PRIMER_RIGHT_0_TM=58.738
PRIMER_INTERNAL_0_TM=57.454
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=44.444
PRIMER_INTERNAL_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=3.4100
PRIMER_RIGHT_0_END_STABILITY=3.7200
PRIMER_PAIR_0_COMPL_ANY_TH=43.66
PRIMER_PAIR_0_COMPL_END_TH=43.66
PRIMER_PAIR_0_PRODUCT_SIZE=108
PRIMER_PAIR_1_PENALTY=1.286428
PRIMER_LEFT_1_PENALTY=0.024450
PRIMER_RIGHT_1_PENALTY=1.261978
PRIMER_INTERNAL_1_PENALTY=9.546432
PRIMER_LEFT_1_SEQUENCE=TGTGTCTTGTGGGTGTGTCT
PRIMER_RIGHT_1_SEQUENCE=ACACAACAGACACACACACA
PRIMER_INTERNAL_1_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG
PRIMER_LEFT_1=79,20
PRIMER_RIGHT_1=188,20
PRIMER_INTERNAL_1=142,27
PRIMER_LEFT_1_TM=60.024
PRIMER_RIGHT_1_TM=58.738
PRIMER_INTERNAL_1_TM=57.454
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=44.444
PRIMER_INTERNAL_1_SELF_ANY_TH=0.00
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_INTERNAL_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_END_STABILITY=3.4100
PRIMER_RIGHT_1_END_STABILITY=3.7200
PRIMER_PAIR_1_COMPL_ANY_TH=35.43
PRIMER_PAIR_1_COMPL_END_TH=35.43
PRIMER_PAIR_1_PRODUCT_SIZE=110
PRIMER_PAIR_2_PENALTY=1.656507
PRIMER_LEFT_2_PENALTY=0.394528
PRIMER_RIGHT_2_PENALTY=1.261978
PRIMER_INTERNAL_2_PENALTY=9.546432
PRIMER_LEFT_2_SEQUENCE=GTGTCTTGTGGGTGTGTCTG
PRIMER_RIGHT_2_SEQUENCE=ACACAACAGACACACACACA
PRIMER_INTERNAL_2_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG
PRIMER_LEFT_2=80,20
PRIMER_RIGHT_2=188,20
PRIMER_INTERNAL_2=142,27
PRIMER_LEFT_2_TM=59.605
PRIMER_RIGHT_2_TM=58.738
PRIMER_INTERNAL_2_TM=57.454
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=44.444
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_END_STABILITY=3.5100
PRIMER_RIGHT_2_END_STABILITY=3.7200
PRIMER_PAIR_2_COMPL_ANY_TH=42.98
PRIMER_PAIR_2_COMPL_END_TH=42.98
PRIMER_PAIR_2_PRODUCT_SIZE=109
PRIMER_PAIR_3_PENALTY=2.382988
PRIMER_LEFT_3_PENALTY=1.121010
PRIMER_RIGHT_3_PENALTY=1.261978
PRIMER_INTERNAL_3_PENALTY=9.546432
PRIMER_LEFT_3_SEQUENCE=GTCTTGTGGGTGTGTCTGTT
PRIMER_RIGHT_3_SEQUENCE=ACACAACAGACACACACACA
PRIMER_INTERNAL_3_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG
PRIMER_LEFT_3=82,20
PRIMER_RIGHT_3=188,20
PRIMER_INTERNAL_3=142,27
PRIMER_LEFT_3_TM=58.879
PRIMER_RIGHT_3_TM=58.738
PRIMER_INTERNAL_3_TM=57.454
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=44.444
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_END_STABILITY=3.1600
PRIMER_RIGHT_3_END_STABILITY=3.7200
PRIMER_PAIR_3_COMPL_ANY_TH=45.65
PRIMER_PAIR_3_COMPL_END_TH=45.65
PRIMER_PAIR_3_PRODUCT_SIZE=107
PRIMER_PAIR_4_PENALTY=2.425217
PRIMER_LEFT_4_PENALTY=0.024450
PRIMER_RIGHT_4_PENALTY=2.400767
PRIMER_INTERNAL_4_PENALTY=9.546432
PRIMER_LEFT_4_SEQUENCE=TGTCTTGTGGGTGTGTCTGT
PRIMER_RIGHT_4_SEQUENCE=TACACAACAGACACACACACA
PRIMER_INTERNAL_4_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG
PRIMER_LEFT_4=81,20
PRIMER_RIGHT_4=189,21
PRIMER_INTERNAL_4=142,27
PRIMER_LEFT_4_TM=60.024
PRIMER_RIGHT_4_TM=58.599
PRIMER_INTERNAL_4_TM=57.454
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=44.444
PRIMER_INTERNAL_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_INTERNAL_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_END_STABILITY=3.4100
PRIMER_RIGHT_4_END_STABILITY=3.7200
PRIMER_PAIR_4_COMPL_ANY_TH=43.66
PRIMER_PAIR_4_COMPL_END_TH=43.66
PRIMER_PAIR_4_PRODUCT_SIZE=109
=