SEQUENCE_ID=exon1
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_INTERNAL_MIN_TM=50
PRIMER_INTERNAL_MAX_TM=70
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
SEQUENCE_EXCLUDED_REGION=100,150
PRIMER_PRODUCT_SIZE_RANGE=200-250
PRIMER_MAX_TM=70
PRIMER_OPT_TM=55
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=12
PRIMER_MIN_TM=50
PRIMER_PAIR_MAX_DIFF_TM=3
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
=
SEQUENCE_ID=exon2
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
=
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_ID=exon2
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
=
SEQUENCE_ID=exon1_2
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_INTERNAL_MIN_TM=50
PRIMER_INTERNAL_MAX_TM=70
PRIMER_EXPLAIN_FLAG=1
SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
SEQUENCE_EXCLUDED_REGION=100,150
PRIMER_PRODUCT_SIZE_RANGE=200-250
PRIMER_MAX_TM=70
PRIMER_OPT_TM=55
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=12
PRIMER_MIN_TM=50
PRIMER_PAIR_MAX_DIFF_TM=3
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=16
=
SEQUENCE_ID=exon2_2
PRIMER_NUM_RETURN=7
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
=
PRIMER_NUM_RETURN=5
PRIMER_MAX_LIBRARY_MISPRIMING=9
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_ID=exon2_3
SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT
SEQUENCE_EXCLUDED_REGION=100,145
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=10
=