Biochemical Properties

Protein Name: neutral protease B

Strain: Bacillus cereus E33L

Number of amino acids: 563

Molecular weight: 62404.4

Theoretical pI: 4.87

Total number of negatively charged residues (Asp + Glu): 74

Total number of positively charged residues (Arg + Lys): 50

Instability index:The instability index (II) is computed to be 43.97

This classifies the protein as unstable

Aliphatic index: 89.09

Grand average of hydropathicity (GRAVY): -0.271

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
9.0	1.4	6.3	6.5	0.0	3.1	7.2	9.6	2.7	4.7	5.8	9.7	1.6	4.3	2.7	5.4	5.6	1.1	5.4	7.8

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
2736	4190	720	850	9