Biochemical Properties

Protein Name: pullulanase

Strain: Bacillus cereus E33L

Number of amino acids: 265

Molecular weight: 30996.6

Theoretical pI: 5.05

Total number of negatively charged residues (Asp + Glu): 37

Total number of positively charged residues (Arg + Lys): 26

Instability index:The instability index (II) is computed to be 45.07

This classifies the protein as unstable

Aliphatic index: 103.77

Grand average of hydropathicity (GRAVY): -0.314

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
5.2	4.3	5.6	5.0	0.7	2.9	9.0	7.0	3.5	6.9	9.4	6.7	2.4	3.8	3.6	4.5	6.5	1.4	5.0	6.5

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
3677	5695	975	1089	22