Biochemical Properties

Protein Name: DNA topoisomerase I

Strain: Chromohalobacter salexigens DSM 3043

Number of amino acids: 877

Molecular weight: 98830.7

Theoretical pI: 6.28

Total number of negatively charged residues (Asp + Glu): 141

Total number of positively charged residues (Arg + Lys): 135

Instability index:The instability index (II) is computed to be 42.52

This classifies the protein as unstable

Aliphatic index: 71.17

Grand average of hydropathicity (GRAVY): -0.669

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
10.3	8.4	2.3	6.5	1.6	3.9	9.6	6.6	1.6	4.2	7.8	7.0	2.1	3.4	5.1	5.0	5.6	1.0	3.2	4.9

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
4329	6873	1251	1335	32