Biochemical Properties

Protein Name: Nitrogenase MoFe cofactor biosynthesis protein NifE [Azotobacter vinelandii DJ]

Strain: Azotobacter Vinelandii

Number of amino acids: 475

Molecular weight: 51884.3

Theoretical pI: 5.59

Total number of negatively charged residues (Asp + Glu): 65

Total number of positively charged residues (Arg + Lys): 57

Instability index:The instability index (II) is computed to be 38.99

This classifies the protein as stable

Aliphatic index: 86.93

Grand average of hydropathicity (GRAVY): -0.209

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
9.8	14.3	0.8	9.4	1.6	3.3	8.2	4.1	1.2	4.1	12.3	0.8	2.5	2.9	6.6	3.7	2.9	0.8	2.0	8.6

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
2283	3639	645	689	23