Biochemical Properties

Protein Name: THII_AZOVD RecName: Full tRNA sulfurtransferase; AltName: Full Sulfur carrier protein ThiS sulfurtransferase; AltName: Full Thiamine biosynthesis protein ThiI; AltName: Full tRNA 4-thiouridine synthase

Strain: Azotobacter Vinelandii

Number of amino acids: 484

Molecular weight: 54732.4

Theoretical pI: 6.64

Total number of negatively charged residues (Asp + Glu): 65

Total number of positively charged residues (Arg + Lys): 62

Instability index:The instability index (II) is computed to be 45.73

This classifies the protein as unstable

Aliphatic index: 99.88

Grand average of hydropathicity (GRAVY): -0.115

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
13.6	6.1	3.8	4.5	1.9	1.9	6.8	7.6	1.1	6.4	11.7	4.2	3.4	1.1	5.3	3.4	6.4	0.4	2.7	7.6

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
2423	3903	699	694	25