Biochemical Properties


Protein Name: RF3_AZOVD RecName: Full Peptide chain release factor 3; Short RF-3

Strain: Azotobacter Vinelandii


Number of amino acids: 527

Molecular weight: 59686.3

Theoretical pI: 5.65

Total number of negatively charged residues (Asp + Glu): 84

Total number of positively charged residues (Arg + Lys): 68

Instability index:The instability index (II) is computed to be 32.68

This classifies the protein as stable

Aliphatic index: 87.72

Grand average of hydropathicity (GRAVY): -0.276

Amino acid composition (%):

Ala Arg Asn Asp Cys Gln Glu Gly His Ile Leu lys Mcf Phe Pro Ser Thr Trp Tyr Val
12.2 9.7 2.8 6.4 0.6 4.2 11.1 7.2 2.5 4.7 9.7 3.6 1.7 1.9 3.3 4.4 3.9 0.6 3.3 6.1


Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:
Carbon Hydrogen Nitrogen Oxygen Sulphur
2669 4194 734 780 20