Biochemical Properties

Protein Name: HNH nuclease

Strain: Halorubrum saccharovorum DSM 1137

Number of amino acids: 114

Molecular weight: 29259.3

Theoretical pI: 5.58

Total number of negatively charged residues (Asp + Glu): 42

Total number of positively charged residues (Arg + Lys): 32

Instability index:The instability index (II) is computed to be 36.88

This classifies the protein as stable

Aliphatic index: 62.46

Grand average of hydropathicity (GRAVY): -0.943

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
6.6	9.0	3.5	7.4	1.6	7.0	9.0	8.2	4.3	3.5	5.9	3.5	1.2	3.1	6.2	4.7	3.9	2.3	2.3	6.6

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
1272	1957	389	396	7