Biochemical Properties

Protein Name: MBHL_AZOVI RecName: Full Uptake hydrogenase large subunit; AltName: Full Hydrogenase subunit alpha; AltName: Full Hydrogenlyase; AltName: Full Membrane-bound hydrogenase large subunit

Strain: Azotobacter Vinelandii

Number of amino acids: 602

Molecular weight: 66736.7

Theoretical pI: 6.16

Total number of negatively charged residues (Asp + Glu): 73

Total number of positively charged residues (Arg + Lys): 63

Instability index:The instability index (II) is computed to be 32.81

This classifies the protein as stable

Aliphatic index: 87.82

Grand average of hydropathicity (GRAVY): -0.294

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
8.4	4.8	4.8	5.7	1.7	2.1	7.8	7.8	2.7	6.7	9.1	5.9	3.2	4.2	5.1	3.8	5.3	0.8	2.9	7.2

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
2979	4635	837	874	18