Biochemical Properties

Protein Name: Hef nuclease

Strain: Halorubrum lacusprofundi ATCC 49239

Number of amino acids: 836

Molecular weight: 91242.2

Theoretical pI: 4.33

Total number of negatively charged residues (Asp + Glu): 182

Total number of positively charged residues (Arg + Lys): 89

Instability index:The instability index (II) is computed to be 32.30

This classifies the protein as stable

Aliphatic index: 79.33

Grand average of hydropathicity (GRAVY): -0.502

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
10.3	6.8	1.9	11.5	0.6	3.3	10.3	7.4	1.3	3.0	7.8	3.8	2.0	2.8	2.6	6.1	6.9	0.4	1.8	9.3

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
3900	6222	1108	1369	22