Biochemical Properties

Protein Name: NAD-dependent DNA ligase LigA

Strain: Halorubrum lacusprofundi ATCC 49239

Number of amino acids: 710

Molecular weight: 77225.9

Theoretical pI: 4.29

Total number of negatively charged residues (Asp + Glu): 148

Total number of positively charged residues (Arg + Lys): 67

Instability index:The instability index (II) is computed to be 37.15

This classifies the protein as stable

Aliphatic index: 81.25

Grand average of hydropathicity (GRAVY): -0.408

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
12.4	8.5	2.5	10.4	0.8	1.4	10.4	8.0	1.4	3.1	8.2	1.0	0.7	3.5	6.6	3.7	5.6	0.8	2.3	8.6

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
3364	5235	951	1117	11