Biochemical Properties

Protein Name: plasmid replication protein RepH

Strain: Halorubrum lacusprofundi ATCC 49239

Number of amino acids: 1002

Molecular weight: 111400.8

Theoretical pI: 4.75

Total number of negatively charged residues (Asp + Glu): 166

Total number of positively charged residues (Arg + Lys): 101

Instability index:The instability index (II) is computed to be 51.51

This classifies the protein as unstable

Aliphatic index: 84.05

Grand average of hydropathicity (GRAVY): -0.474

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
8.3	7.5	2.0	8.6	0.6	3.5	8.0	7.6	2.5	5.3	10.3	2.6	1.4	2.0	4.8	8.0	6.8	2.1	3.1	5.2

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
4882	7643	1379	1569	20