Biochemical Properties

Protein Name: ATP-dependent DNA helicase PcrA

Strain: Bacillus cereus G9241

Number of amino acids: 747

Molecular weight: 84477.3

Theoretical pI: 6.59

Total number of negatively charged residues (Asp + Glu): 105

Total number of positively charged residues (Arg + Lys): 103

Instability index:The instability index (II) is computed to be 34.63

This classifies the protein as stable

Aliphatic index: 89.44

Grand average of hydropathicity (GRAVY): -0.375

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
7.9	6.4	4.8	5.8	0.3	3.5	8.3	5.8	1.2	7.2	8.7	7.4	2.3	3.9	2.8	5.9	7.0	0.9	3.3	6.7

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
3754	6007	1033	1141	19