Biochemical Properties

Protein Name: DNA topoisomerase I

Strain: Bacillus cereus G9241

Number of amino acids: 692

Molecular weight: 79078.0

Theoretical pI: 8.51

Total number of negatively charged residues (Asp + Glu): 105

Total number of positively charged residues (Arg + Lys): 112

Instability index:The instability index (II) is computed to be 39.79

This classifies the protein as stable

Aliphatic index: 77.88

Grand average of hydropathicity (GRAVY): -0.544

Amino acid composition (%):

Ala	Arg	Asn	Asp	Cys	Gln	Glu	Gly	His	Ile	Leu	lys	Mcf	Phe	Pro	Ser	Thr	Trp	Tyr	Val
5.9	5.8	3.8	4.9	1.9	3.9	10.3	5.6	0.9	6.1	6.8	10.4	2.9	4.2	4.6	4.9	5.8	0.9	3.0	7.5

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 Hours (Mammalian reticulocytes, in vitro)

Atom composition:

Carbon	Hydrogen	Nitrogen	Oxygen	Sulphur
3510	5625	955	1051	33